Protein_Domain

Part:BBa_K4643010:Design

Designed by: Baptiste Rivoirard   Group: iGEM23_Sorbonne-U-Paris   (2023-09-27)


Cytochrome P450 52-M1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 619
    Illegal EcoRI site found at 796
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 908
    Illegal PstI site found at 1504
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 619
    Illegal EcoRI site found at 796
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 908
    Illegal PstI site found at 1504
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 619
    Illegal EcoRI site found at 796
    Illegal EcoRI site found at 1369
    Illegal BglII site found at 308
    Illegal BamHI site found at 1278
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 619
    Illegal EcoRI site found at 796
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 908
    Illegal PstI site found at 1504
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 619
    Illegal EcoRI site found at 796
    Illegal EcoRI site found at 1369
    Illegal PstI site found at 908
    Illegal PstI site found at 1504
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 66


Design Notes

The original sequence has been modify by our codon optimisation algorithm (cf our contribution page on our wiki)


Source

genomic sequence from Starmerella bombicola (Yeast) (Candida bombicola)

References